Role of Butyryl Phosphate in the Energy Metabolism of Clostridium Tetanomorphum.

Purification and role of phosphotransbutyrylase.

In recent years acyl phosphates have received much attention. For example, acetyl (1, 2), propionyl (3, 4), butyryl (5, 6), carbamyl (7, 8), and aspartyl (9) phosphates have been studied in detail. In fermentative bacteria the role of phosphotransacetylase, which facilitates an interconversion of acetyl phosphate and acetyl coenzyme A has been well documented (1,2,10-12). An enzyme capable of c...

Deferred metabolism of glucose by Clostridium tetanomorphum.

Phosphotransbutyrylase from Clostridium acetobutylicum ATCC 824 and its role in acidogenesis.

Phosphotransbutyrylase (phosphate butyryltransferase [EC 2.3.1.19]) from Clostridium acetobutylicum ATCC 824 was purified approximately 200-fold to homogeneity with a yield of 13%. Steps used in the purification procedure were fractional precipitation with (NH4)2SO4, Phenyl Sepharose CL-4B chromatography, DEAE-Sephacel chromatography, high-pressure liquid chromatography with an anion-exchange c...

Allostery of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase in Clostridium: another conserved generic characteristic.

Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. acetobutylicum, and C. tetanomorphum. Quantitative differences in the enzymolog...

Threonine Deaminase of Clostridium tetanomorphum

Threonine deaminase (Lthreonine hydrolyase (deaminating), EC 4.2.1.16) has been purified approximately 700-fold from extracts of Clostridium tetanomorphum. Both threonine and serine can serve as substrates, but threonine is deaminated 5 to 10 times more rapidly than serine. Pyridoxal phosphate, a reducing agent, and alkaline pH are required for the deamination of either amino acid. A plot of th...